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KMID : 0438219750120010227
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Korea University Medical Journal
1975 Volume.12 No. 1 p.227 ~ p.234
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Effects of Substrates of the Tricarboxylic Acid Cycle on the Oxidation by Rat Brain Slices
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Abstract
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The purpose of the present experiment was to determine the effects of intermediate metabolites of Krebs cycle on brain metabolism.
Oxygen consumption of cortex, thalamus, hypothalamus, medulla oblongata and cerebellum of rat brain was measured in Krebs-Ringer bicarbonate buffer solution (pH. 7.4) and the same solutions mixed with 10mM/liter of citric acid, a-ketoglutaric acid, fumaric acid, and malic acid respectively, with or without addition of glucose. Direct method of Warburg was used for the measurement of oxygen consumption throughout the experiment.
Oxygen consumption was significantly increased in all medium when-intermediates was added, and in order of succinic acid, a-ketoglutaric acid, fumaric acid, malic acid and citric acid.
Glucose enhanced the oxygen consumption in solutions containing citric acid, fumaric acid, malic acid while suppressed in the solution mixed with succinic acid, in all areas of the rat brain. In solution mixed with a-ketoglutaric acid, oxygen consumption was accelerated only in cortex and medulla oblongata by addition of glucose.
In general, the rat brain tissue is capable of utilizing intermediatemetabolites of Krebs cycle in the absence of glucose and the metabolism is significantly accelerated by glucose in all but succinic acid containing solutions, suggesting a permissive. action of glucose on Krebs cycle intermediate utilization.
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